Paracoccidioides brasiliensis PbP27 gene: knockdown procedures and functional characterization
Paracoccidioides brasiliensis PbP27 gene encodes a protein localized in both the fungal
cytoplasm and cell wall. The parasitic infectious form produces this protein preferentially with
the gene's expression varying between the fungus phylogenetic species. The biological
function of the native p27 has yet to be determined during either growth of the yeast or host
infection. Therefore, in this study, through the use of antisense RNA technology and
Agrobacterium tumefaciens-mediated transformation, we generated mitotically stable PbP27 …
cytoplasm and cell wall. The parasitic infectious form produces this protein preferentially with
the gene's expression varying between the fungus phylogenetic species. The biological
function of the native p27 has yet to be determined during either growth of the yeast or host
infection. Therefore, in this study, through the use of antisense RNA technology and
Agrobacterium tumefaciens-mediated transformation, we generated mitotically stable PbP27 …
Paracoccidioides brasiliensis PbP27 gene: knockdown procedures and functional characterization
IP Torres Gómez, O Hernández Ruiz… - 2014 - bibliotecadigital.udea.edu.co
Paracoccidioides brasiliensis PbP27 gene encodes a protein localized in both the fungal
cytoplasm and cell wall. The parasitic infectious form produces this protein preferentially with
the gene's expression varying between the fungus phylogenetic species. The biological
function of the native p27 has yet to be determined during either growth of the yeast or host
infection. Therefore, in this study, through the use of antisense RNA technology and
Agrobacterium tumefaciens-mediated transformation, we generated mitotically stable PbP27 …
cytoplasm and cell wall. The parasitic infectious form produces this protein preferentially with
the gene's expression varying between the fungus phylogenetic species. The biological
function of the native p27 has yet to be determined during either growth of the yeast or host
infection. Therefore, in this study, through the use of antisense RNA technology and
Agrobacterium tumefaciens-mediated transformation, we generated mitotically stable PbP27 …
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