One the way to increase the population of native chickens is by applying the artificial insemination (IB) method. The purpose of this study was to determine the effect of the dose level of the infected spermatozoa on the level of fertility and hatchability of free–range chicken eggs. The purpose of this IB dose, in order to determine the level of comparison of the most efficient rooster and hen in a maintenance. For rooster, the semen is collected, then the cement is mixed from 3 male free–range chickens aged about 12–15 months. The known concentration of sperm spermatozoa is divided into 3 parts, then the semen is then diluted with physiological NaCl until the final dose is obtained as follows, 50 x 10 6/0, 1 ml is treatment dose of 1, 100 x 10 6/0, 1 ml is the treatment dose 2, 200 x 10 6/0, 1 ml is the treatment dose 3. Then, 15 female free–range chickens were inseminated, in order to find out how the dose affects fertility and hatchability. The hens used for IB are hens aged 12–18 months. The results showed that the spermatozoa dose treatment had an effect on the fertility and hatchability of native chicken eggs. For each treatment dose 1, 2, dan 3, the egg fertility was 52±10.95%; 72.00±17.89%; and 80.00±14.14%, while the outermost hatchability, the percentage was 44, 00±16.73%; 60.00±14.14%; and 72.00±10.95%. The conclusion of this study is that the dose of sperm has an effect on fertility and hatchability of native chicken eggs.