Prayer Dhuha when implemented in a County and tuma’ninah, will be two movements of muscle contraction isometric and isotonic muscular contractions. Movement with isometric and isotonic muscle contraction both can decrease blood glucose levels. This research aims to prove that Dhuha Prayer can decrease blood glucose levels. This research design uses “a randomized control group pre test-post test design”, with independent variables is Dhuha Prayer and for the dependent variables is the blood glucose levels. The sample size in this study were 15 respondents respectively for the control and treatment groups. Samples are taken using simple random sampling. Statistical test use of SPSS with T-test, with significance level a? 0, 05. Test results of Independent T-testn, before