There are many studies dedicated to phytochemistry of Vitex agnus-castus and V. cannabifolia growing in their endemic conditions, but there is no available information about constituents of ones growing in temperate climates. Also the data of phytochemical composition of stems and inflorescences are insufficient. The TLC and HPLC methods were used for identification and quantification, while UV-spectrophotometry was used for estimation of sums of flavonoids, polyphenols and cinnamic acids in stems, inflorescences and leaves. The content of caffeic acid was much lower that it was reported for raw material collected in Turkey. The highest total polyphenols content was found in inflorescences and leaves of each plant (up to 6.65%). The highest total flavonoids content was in V. agni-casti inflorescences (7.59%). In the rest of raw material types it was no higher than 2.23%. The total cinnamic acids content is higher in V. cannabifoliae raw material (up to 6.10%).