Buffalo milk due to its high fat content from the energy point of view is more valuable compared to cow's milk. The percentage of fat varies depending on the stage of lactation, season, individual, health, animal age and diet. The study of the chemical composition and the antioxidant capacity of buffalo milk is required for both scientific and technological considerations, given the importance of this type of milk in the consumer's diet. The purpose of this study was to evaluate the antioxidant capacity and the physico-chemical composition of buffalo milk by lactation. Buffalo milk can have different qualities, so the quality of buffalo milk is also determined by its content in its components (protein, fat, lactose, vitamins, fatty acids, water content, antioxidant capacity). The physicochemical parameters and antioxidant capacity were influenced by lactation, presenting the highest values in lactations III and IV. During lactation I, parameters such as fat, protein and lactose showed a content of 7.88, 4.35 and 4.71%, respectively. Furthermore, in lactation IV, fat, protein and lactose had increased, showing the content of these parameters as follows 9.53, 4.68 and 4.77%. Antioxidant capacity of buffalo milk showed the highest numbers in lactation III (360.1) and IV, 358.9 μg/ml. From all of the analyzed parameters, only total dry substance content presented the most increased values 18.9% in lactation I.