Carotenoid pigments are natural dyes that have an influence on organism. For the human body, carotenoids function as antioxidants, anticancer, anti-bacteria and help maintain eye health. Carotenoid pigments are found in groups of bacteria, fungi, molds, algae and green plants and one of them in crustaceans is the crab Ozius sp. To determine the metabolic process of carotenoid pigments, each crab organ of Ozius sp. isolated using thin layer chromatography separation method to determine the type of pigment contained therein. The type of pigment identified in the crab Ozius sp. females with the TLC method namely: β-carotene, Cantanxhantine, and β-cryptosanthin.