… The first step of preparing plant single‐cell suspension usually starts with digestion of cell
wall via cellulase and hemicellulase. In PsctH, we compared and evaluated different protocols
used to prepare protoplasts and offer a feasible and efficient experimental pipeline for
reference, which included preparation and isolation of tissue samples, enzyme digestion,
purification, and the detection of the integrity of single cells (Figure 1f). To standardize and link
the process of plant single‐cell data mining, a flexible pipeline of plant single‐cell transcriptome …