Background: To detect and type plasmids responsible for penicillin and tetracycline
resistance in Neisseria gonorrhoeae isolates using a novel duplex polymerase chain
reaction (PCR) assay. Methods: A duplex PCR assay, to detect and type penicillinase-
producing N. gonorrhoeae (PPNG), and plasmid-mediated tetracycline resistant N.
gonorrhoeae (TRNG), was developed on the basis of published single assays. Gonococcal
Isolate Surveillance Project control strains were used in assay development and then 209 …

Background Treatment of gonorrhea is complicated by the development of antimicrobial
resistance in Neisseria gonorrhoeae (GC) to the antibiotics recommended for treatment.
Knowledge on types of plasmids and the antibiotic resistance genes they harbor is useful in
monitoring the emergence and spread of bacterial antibiotic resistance. In Kenya, studies on
gonococcal antimicrobial resistance are few and data on plasmid mediated drug resistance
is limited. The present study characterizes plasmid mediated resistance in N. gonorrhoeae …