This study was carried out to determine the hematological changes (TEC, Hb, PCV) and biochemical changes (AST/SGOT, ALT/SGPT and Ca), in commercial broiler chickens due to the use of Probiotics (protexin). Total 60, day old of vencobb broiler chicks was used for the experiment. The chicks were distributed into four dietary treatments having three replications in each treatment. For hematological examination, 5 ml blood sample was collected from wing vein of each group by and kept in vials containing anticoagulant (sodium-EDTA) and this was done on day of 7, 14, 21, 28 and 35 during experimental period. Biochemical parameters, SGOT and SGPT were determined by the use of the specific test kit and analyzer (3000 evolution) and Ca by RX Monza analyzer. Probiotic effects were found at 35 days old broilers statistically significant at (P< 0.01) level of significance for TEC, PCV, Hb, SGOT and Caw here as SGPT was at (P< 0.05) level of significance. Probiotics perhaps increase the beneficial microorganisms and decreases the pathogenic microorganisms in the gastrointestinal tract.