Potential of pmoA Amplicon Pyrosequencing for Methanotroph Diversity Studies
C Lüke, P Frenzel - Applied and environmental microbiology, 2011 - Am Soc Microbiol
C Lüke, P Frenzel
Applied and environmental microbiology, 2011•Am Soc MicrobiolWe analyzed the potential of pmoA amplicon pyrosequencing compared to that of Sanger
sequencing with paddy soils as a model environment. We defined operational taxonomic
unit (OTU) cutoff values of 7% and 18%, reflecting methanotrophic species and major
phylogenetic pmoA lineages, respectively. Major lineages were already well covered by
clone libraries; nevertheless, pyrosequencing provided a higher level of diversity at the
species level.
sequencing with paddy soils as a model environment. We defined operational taxonomic
unit (OTU) cutoff values of 7% and 18%, reflecting methanotrophic species and major
phylogenetic pmoA lineages, respectively. Major lineages were already well covered by
clone libraries; nevertheless, pyrosequencing provided a higher level of diversity at the
species level.
Abstract
We analyzed the potential of pmoA amplicon pyrosequencing compared to that of Sanger sequencing with paddy soils as a model environment. We defined operational taxonomic unit (OTU) cutoff values of 7% and 18%, reflecting methanotrophic species and major phylogenetic pmoA lineages, respectively. Major lineages were already well covered by clone libraries; nevertheless, pyrosequencing provided a higher level of diversity at the species level.
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