Single and inactivated combined vaccines were prepared against duck Pasteurellosis and Riemerella anatipestifer infection in ducks. All the prepared vaccine were checked for sterility and found to be free from foreign contaminants. Also, all were safe for vaccinated birds (showing no post vaccination sickness) and were immunogenic inducing high levels of specific antibodies against both of P. multocida (A5 and D2) and R. anatipestifer strains (RA1 and RA2). Indirect hemagglutination (IHA and ELISA techniques) revealed that such vaccines induced a satisfactory antibody response when used as primary vaccination ducks. Improved antibody response was recorded till 6 month when the vaccines were given in two doses at three weeks intervals. There is no antagonizing effect between the used antigens in the combined vaccine on the duck's immune responses. The protective potential efficacy of the prepared vaccines was also measured by determining the rate of protection of the birds of each vaccinated group by challenge exposure at 3 weeks post booster vaccination.