The objective of the present study was to determine the prevalence of Dirofilaria immitis (DI) in domestic canines in the municipalities of Cuautepec and Acapulco de Juárez, Guerrero, Mexico. Cluster sampling was used according to the selected addresses. Blood samples were obtained only from dogs older than one year of the two municipalities. In the study, 15.68% were found in the municipality of Cuautepec and 7.44% in domestic canines positive for microfilariae in Acapulco de Juárez. The highest percentage of positive animals was found in males, in animals> 3 years of age and small and medium animals in Cuautepec municipality (P< 0.05). Likewise, in the municipality of Acapulco de Juárez, a higher percentage of positive females was found, with an age> 3 years of age, of an indefinite mestizo race and living outside the home (P< 0.05). It is concluded that domestic canines in the municipalities of Cuautepec and Acapulco de Juárez, Guerrero, found a high prevalence of DI, and males had a higher percentage in the municipality of Cuautepec and females in Acapulco de Juárez.