Prevalence of E.coli among poultry farms was investigated in these studies, which cover differentprovinces in Egypt through examination of 400 chicken samples using standard methods for isolationand identification of E.coli. The result showed presence of E.coli among imported chicks in rate 44%and from local broiler chickens about 75%. The incidence of E.coli in on day old living diseased chickswas(58.3), while the incidence of E.coli from local broiler chickens was (71%) from living diseasedchickens and about (83%) from freshly dead chickens These serotype of E.coli were O63, O103, O125,O158, O44. Antibiogram pattern test indicated the highest rate of resistant against tetracycline groupwhere about 80% followed by the2 β-Lactam antibiotic (73.3%), erythromycin about 63.3% of testedisolate were resistant, florfenicol about 53.3% were resistant , gentamycin was about 46.6% wereresistance , finally ciprofloxacin about 40% of tested isolate were resistant by using disc diffusionmethod