The antidiabetic activity of Ethanol extract of leaves of Argyriea cuneata was evaluated in alloxan-induced diabetic rats. The various parameters like fasting serum glucose, body weight, total cholesterol, triglycerides, high density lipoprotein, total protein and oral glucose tolerance test were studied. The results were found to be significant antidiabetic by reducing the serum glucose level, improving body weight, attenuating the altered lipid profile toward the normal by reducing the elevated total cholesterol and triglycerides, and increasing the high density lipoprotein and total protein level. Therefore the present study justifies that the ethanol extract of Argyriea cuneata exhibits significant antidiabetic activity.