Biogas is one of the important sources of sustainable and green energy. The generation of biogas using kitchen waste as main constituent and cow manure as cosubstrate has been the main aim while preceding the experiment. Anaerobic digesters (AD) are used for the gas generation purpose. A temperature range of 30-35 C is maintained to facilitate the mesophillic conditions. The pH of the slurry is checked and maintained on weekly basis. The gas production is recorded with a retention time of 20 days. The methane content is measured by using Syringe protocol. 0.05196 m3 of gas have been produced by the experiment. The digested sludge can be used as a significant resource of natural fertilizer. The experiment yields important results, which suggested that the kitchen waste and cow manure co-digestion, can produce good biogas with methane content of about 60%. The work presented herein provides imminent information for future design and operation of micro and macro plants.