Production of single contracting human induced pluripotent stem cell‐derived cardiomyocytes: matrigel mattress technique
Current protocols in stem cell biology, 2017•Wiley Online Library
This unit describes the published Matrigel mattress method. Briefly, we describe the
preparation of the mattress, replating of the human induced pluripotent stem cell–derived
cardiomyocytes (hiPSC‐CM) on the Matrigel mattress, and hiPSC‐CM mattress
maintenance. Adherence to this protocol will yield individual, robustly shortening hiPSC‐
CMs, which can be used for downstream applications.© 2017 by John Wiley & Sons, Inc.
preparation of the mattress, replating of the human induced pluripotent stem cell–derived
cardiomyocytes (hiPSC‐CM) on the Matrigel mattress, and hiPSC‐CM mattress
maintenance. Adherence to this protocol will yield individual, robustly shortening hiPSC‐
CMs, which can be used for downstream applications.© 2017 by John Wiley & Sons, Inc.
Abstract
This unit describes the published Matrigel mattress method. Briefly, we describe the preparation of the mattress, replating of the human induced pluripotent stem cell–derived cardiomyocytes (hiPSC‐CM) on the Matrigel mattress, and hiPSC‐CM mattress maintenance. Adherence to this protocol will yield individual, robustly shortening hiPSC‐CMs, which can be used for downstream applications. © 2017 by John Wiley & Sons, Inc.
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