This study aimed at investigating the psychological, physiological, performance and perceptive responses to a regional level Brazilian jiu-jitsu competition. The study included 12 Brazilian jiu-jitsu athletes graded blue belt. The psychological responses were measured by the Profile of Mood States–POMS and Sport Competition Anxiety Test–SCAT. Glucose, β-hydroxybutyrate and lactate were determined from blood samples collected at rest and immediately after the fights at the earlobe. Saliva samples were also collected at rest and immediately after the fights to determine cortisol and immunoglobulin A (IgA). Maximal isometric grip strength was tested using a handgrip dynamometer, before and after the fights. Rating of perceived exertion was also assessed after the fights using the 6-20 Borg scale. The athletes were asked to indicate on an anatomical diagram of the anterior and posterior views of the body, the areas they perceived to have experienced most exertion during fights. Before the competition the athletes presented an iceberg profile of mood state and medium competitive anxiety. As a result of the fights, glucose, lactate, cortisol and IgA increased significantly. However, β-hydroxybutyrate level remained constant after the fights. Physical exertion during the match resulted in significant reductions only in the dominant handgrip strength. In addition, the athletes rated the fight as hard and very hard and indicated the forearm, wrist and frontal thigh as the principal points of fatigue. The results indicated that Brazilian jiu-jitsu fights exert a moderate demand on the glycolytic pathway and caused significant reductions in handgrip strength.