The purpose of the research is to analyze the relationship between rail service quality and customer satisfaction. A total of 356 sets of questionnaires were distributed via email to passengers of rail transport in Malaysia and a total of 258 questionnaires were usable for the analysis. Using Pearson correlation analysis and multiple regression analysis our research found that only four rail service quality dimensions namely reliability, responsiveness, convenience, and connection are significant in predicting customer satisfaction. For future research, it is suggested that researchers use a bigger sample size and qualitative analysis for in-depth analysis.