[HTML][HTML] Recruitment of RNA Polymerase Is a Rate-limiting Step for the Activation of the ς54 Promoter Pu ofPseudomonas putida
The activity of the ς 54-promoter Pu of Pseudomonas putida was examined in vitro with a
DNA template lacking upstream activating sequences, such that RNA polymerase can be
activated by the enhancer-binding protein XylR only from solution. Although the transcription
activation pathway in this system lacked the step of integration host factor (IHF)-mediated
looping of the XylR· DNA complex toward the prebound RNA polymerase, IHF still
stimulated promoter activity. The positive effect of IHF became evident not only with XylR …
DNA template lacking upstream activating sequences, such that RNA polymerase can be
activated by the enhancer-binding protein XylR only from solution. Although the transcription
activation pathway in this system lacked the step of integration host factor (IHF)-mediated
looping of the XylR· DNA complex toward the prebound RNA polymerase, IHF still
stimulated promoter activity. The positive effect of IHF became evident not only with XylR …
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