Removal of PCR inhibitors from human faecal samples through the use of an aqueous two-phase system for sample preparation prior to PCR
PG Lantz, M Matsson, T Wadström… - Journal of Microbiological …, 1997 - Elsevier
A sample preparation method based on a two-phase aqueous polymer system, composed of
8%(w/w) polyethylene glycol 4000 and 11%(w/w) dextran 40, was employed to remove PCR-
inhibitory substances from human faeces prior to PCR. The majority of the PCR-inhibitory
substances, including bile salts, were shown to be distributed in the polyethylene glycol-rich
top phase, whereas target bacteria were detected by PCR in the dextran-rich bottom phase.
The efficiency of the aqueous two-phase system in removing PCR-inhibitory substances …
8%(w/w) polyethylene glycol 4000 and 11%(w/w) dextran 40, was employed to remove PCR-
inhibitory substances from human faeces prior to PCR. The majority of the PCR-inhibitory
substances, including bile salts, were shown to be distributed in the polyethylene glycol-rich
top phase, whereas target bacteria were detected by PCR in the dextran-rich bottom phase.
The efficiency of the aqueous two-phase system in removing PCR-inhibitory substances …
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