… cDNA clone of the SARS-CoV-2 USA-WA1/2020 strain based on the use of a bacterial
artificial chromosome (BAC). Recombinant SARS-CoV-2 (rSARS-CoV-2) was readily rescued by …

… For the rescue of rSARS-CoV-2 from pBeloBAC11-SARS-CoV-2, we recommend three …
the probability of successful rescue. If one is attempting rescue of multiple recombinant viruses, …

… for efficient viral rescue and the SARS-CoV-2 genome is ~… with the use of the SARS-CoV-2
BAC-based RG system, we … assembly of the entire SARS-CoV-2 genome into pBeloBAC and …

… bacterial artificial chromosome (BAC) technology to rescue recombinant SARS-CoV-2
from … and growth kinetics, utilizing recombinant SARS-CoV-2 rescued from BAC. VeroE6/…

… this major limitation, we have developed a bacterial artificial chromosome (BAC)-based
reverse … A schematic representation of the experimental procedures and steps for the rescue of …

… The six DNA fragments covering the full-length SARS-CoV-2 genome and a linker fragment
… 2.4 SARS-CoV-2 rescue To rescue recombinant SARS-CoV-2, BACs or CPER products …

… cDNA clones of SARS-CoV-2, which must be rescued at biosafety level 3 (… Bacterial artificial
chromosome-based SARS-CoV-2 reverse genetic system Bacterial artificial chromosome (…

… —such as cloning in low-copy bacterial artificial chromosomes (BACs) or vaccinia virus, or
… the GFP-encoding SARS-CoV-2 RNAs. Indeed, we could rescue infectious viruses for almost …

… respiratory syndrome CoV-2 (SARS-CoV-2) has already caused substantial … SARS-CoV-2,
we developed a passage-free SARS-CoV-2 clone based on a bacterial artificial chromosome (…

… on bacterial artificial chromosome (BAC). The genomic complementary DNA (cDNA) of
severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2… rescued viruses for SARS-CoV-2 …