[HTML][HTML] Rigorous thermal control during intracytoplasmic sperm injection stabilizes the meiotic spindle and improves fertilization and pregnancy rates
WH Wang, L Meng, RJ Hackett, R Oldenbourg… - Fertility and sterility, 2002 - Elsevier
Objective: To examine the effects of different thermodynamic control systems on the
temperature stability of human eggs during in vitro manipulation, with the integrity of meiotic
spindles imaged using the LC-PolScope (Cambridge Research & Instrumentation, Inc.,
Woburn, MA). Design: We performed intracytoplasmic sperm injection (ICSI) and/or imaging
of eggs with the temperature regulated by three different systems: thermostated coverslip
(system 1), thermostated coverslip combined with objective heater (system 2), and …
temperature stability of human eggs during in vitro manipulation, with the integrity of meiotic
spindles imaged using the LC-PolScope (Cambridge Research & Instrumentation, Inc.,
Woburn, MA). Design: We performed intracytoplasmic sperm injection (ICSI) and/or imaging
of eggs with the temperature regulated by three different systems: thermostated coverslip
(system 1), thermostated coverslip combined with objective heater (system 2), and …
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