… Arrayed CRISPR libraries extend the scope of gene-perturbation screens but require large
… genome-wide arrayed libraries for human gene ablation (19,936 plasmids), activation, and …

… of highly active, versatile CRISPR arrayed libraries that overcome the limitations of existing
… underscore the general robustness of arrayed CRISPR activation screens with the T.gonfio …

… locus, the resulting RNA (crRNA) and trans-activating RNA (… resulting in error-prone repair
with deletions or insertions (INDELs). In … Individual sgRNAs were cloned into a highly versatile …

… pooled or arrayed guide RNA (gRNA) libraries that can … both libraries, interactions between
the deletion and activation targets … After incubation at 30 C for 16 h with vigorous shaking, the …

… a new generation of highly active, robust, generic and versatile CRISPR arrayed libraries …
our arrayed libraries with a novel algorithm to select the optimal combination of four sgRNAs (…

… system when studying the essential genes whose deletion caused a lethal effect. CRISPRi
… a robust and versatile multiplexed gRNA arrayed library for both CRISPR activation and …

… We developed a fluorescence-activated cell sorting (FACS)-based screening method using
pooled CRISPR-Cas9-mediated genome-wide deletion libraries in conjunction with antibody …

… cancer treatments, achieving durable responses in a subset … of T cell suppression and
activation, but systematic strategies … >75,000 guides in the genome-wide (GW) library. Bottom: LFC …

… In arrayed CRISPR screens, different perturbations are … CRISPR gRNA libraries may be
genome-wide or focused on … CRISPRko introduces small insertions and deletions that typically …

… Deletions and chromosomal rearrangements induced by … Arrayed library screening often
requires specialized … Genome-wide Cas9 screens are increasingly popular due to their …