Sea bass ghrelin: molecular cloning and mRNA quantification during fasting and refeeding
G Terova, S Rimoldi, G Bernardini, R Gornati… - General and …, 2008 - Elsevier
Ghrelin is a novel appetite-inducing peptide hormone secreted by the stomach. The purpose
of this study was first to identify the cDNA encoding sequence for ghrelin in sea bass
(Dicentrarchus labrax). Using molecular cloning techniques we sequenced the cDNA
corresponding to sea bass ghrelin mRNA. A total of 798 bases including a 5′-untranslated
region (89bp), an open reading frame (ORF)(324bp), and a 3′-untranslated region (385bp)
were detected. Nucleotide sequence (ORF) encoded a 108 amino acid prepropeptide that …
of this study was first to identify the cDNA encoding sequence for ghrelin in sea bass
(Dicentrarchus labrax). Using molecular cloning techniques we sequenced the cDNA
corresponding to sea bass ghrelin mRNA. A total of 798 bases including a 5′-untranslated
region (89bp), an open reading frame (ORF)(324bp), and a 3′-untranslated region (385bp)
were detected. Nucleotide sequence (ORF) encoded a 108 amino acid prepropeptide that …
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