Selection and Validation of Reference Genes for Quantitative Real-time PCR in Gentiana macrophylla
Y He, H Yan, W Hua, Y Huang, Z Wang - Frontiers in plant science, 2016 - frontiersin.org
Real time quantitative PCR (RT-qPCR or qPCR) has been extensively applied for analyzing
gene expression because of its accuracy, sensitivity, and high throughput. However, the
unsuitable choice of reference gene (s) can lead to a misinterpretation of results. We
evaluated the stability of 10 candidates–five traditional housekeeping genes (UBC21,
GAPC2, EF-1 α 4, UBQ10, and UBC10) and five novel genes (SAND1, FBOX, PTB1, ARP,
and Expressed1)–using the transcriptome data of Gentiana macrophylla. Common statistical …
gene expression because of its accuracy, sensitivity, and high throughput. However, the
unsuitable choice of reference gene (s) can lead to a misinterpretation of results. We
evaluated the stability of 10 candidates–five traditional housekeeping genes (UBC21,
GAPC2, EF-1 α 4, UBQ10, and UBC10) and five novel genes (SAND1, FBOX, PTB1, ARP,
and Expressed1)–using the transcriptome data of Gentiana macrophylla. Common statistical …
Selection and validation of reference genes for quantitative real-time PCR in Gentiana macrophylla.
HYH He YiHan, YHL Yan HaiLing, HWP Hua WenPing… - 2016 - cabidigitallibrary.org
Real time quantitative PCR (RT-qPCR or qPCR) has been extensively applied for analyzing
gene expression because of its accuracy, sensitivity, and high throughput. However, the
unsuitable choice of reference gene (s) can lead to a misinterpretation of results. We
evaluated the stability of 10 candidates-five traditional housekeeping genes (UBC21,
GAPC2, EF-1 α 4, UBQ10, and UBC10) and five novel genes (SAND1, FBOX, PTB1, ARP,
and Expressed1)-using the transcriptome data of Gentiana macrophylla. Common statistical …
gene expression because of its accuracy, sensitivity, and high throughput. However, the
unsuitable choice of reference gene (s) can lead to a misinterpretation of results. We
evaluated the stability of 10 candidates-five traditional housekeeping genes (UBC21,
GAPC2, EF-1 α 4, UBQ10, and UBC10) and five novel genes (SAND1, FBOX, PTB1, ARP,
and Expressed1)-using the transcriptome data of Gentiana macrophylla. Common statistical …
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