Selective inhibitors of JAK1 targeting an isoform-restricted allosteric cysteine

ME Kavanagh, BD Horning, R Khattri, N Roy… - Nature chemical …, 2022 - nature.com
ME Kavanagh, BD Horning, R Khattri, N Roy, JP Lu, LR Whitby, E Ye, JC Brannon, A Parker…
Nature chemical biology, 2022nature.com
The Janus tyrosine kinase (JAK) family of non-receptor tyrosine kinases includes four
isoforms (JAK1, JAK2, JAK3, and TYK2) and is responsible for signal transduction
downstream of diverse cytokine receptors. JAK inhibitors have emerged as important
therapies for immun (onc) ological disorders, but their use is limited by undesirable side
effects presumed to arise from poor isoform selectivity, a common challenge for inhibitors
targeting the ATP-binding pocket of kinases. Here we describe the chemical proteomic …
Abstract
The Janus tyrosine kinase (JAK) family of non-receptor tyrosine kinases includes four isoforms (JAK1, JAK2, JAK3, and TYK2) and is responsible for signal transduction downstream of diverse cytokine receptors. JAK inhibitors have emerged as important therapies for immun(onc)ological disorders, but their use is limited by undesirable side effects presumed to arise from poor isoform selectivity, a common challenge for inhibitors targeting the ATP-binding pocket of kinases. Here we describe the chemical proteomic discovery of a druggable allosteric cysteine present in the non-catalytic pseudokinase domain of JAK1 (C817) and TYK2 (C838), but absent from JAK2 or JAK3. Electrophilic compounds selectively engaging this site block JAK1-dependent trans-phosphorylation and cytokine signaling, while appearing to act largely as ‘silent’ ligands for TYK2. Importantly, the allosteric JAK1 inhibitors do not impair JAK2-dependent cytokine signaling and are inactive in cells expressing a C817A JAK1 mutant. Our findings thus reveal an allosteric approach for inhibiting JAK1 with unprecedented isoform selectivity.
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