… and coworkers employed Ni 2+ chelating peptides that were … we elucidate the optimal
combination of the peptide sequence, … Amide bond hydrolysis was induced by complexation by the …

… in peptide fusion technologies to maximize the protein … protein expression system armed
with variety of cardinal fusion tags, expression of the functionally active recombinant protein in E…

… via combinatorial protein engineering approaches, … /or specificity optimization, modification
of the binding protein via … residues of target proteins in a sequence-specific manner and links …

… may include the peptide bond hydrolysis in X 1 -S/Tc/pHcX 2 motifs by released Ni 2+ ions.
… 150 mM NaCl buffer with or without nickel ions [1 mM Ni(NO 3 ) 2 ] under optimal (pH 8.2, 50C…

… hydrolysis of an extremely stable tertiary peptide bond (Ser–Pro) in the Ser-Pro-Phe sequence
by an artificial enzyme, metal (Pd 2+ , Co 2… The optimized transition state for this process (…

… of how Ni(II) substitution alters the DNA sequence-specific … and catalyze the hydrolysis of
peptide bonds between Gln … in various fields, including protein engineering, medicine, and …

… The carbonyl oxygen of the N-terminal peptide bond of cysteine … is also required for
sequence-specific cleavage by cyanogen … optimization of peptide-segments coupling dependent …

… in the formation of a native amide bond and extrusion of the … sequence-specific introduction
of bioorthogonal tags into a … molecular biology, protein engineering, synthetic chemistry, and …

… (18−22) This problem can be overcome through the incorporation of the active metal salts …
proven to be active toward hydrolysis of peptide bonds in a range of dipeptides and proteins. (…

… One of the goals of protein engineering is the installation of … The active-site cysteine of sortase
A forms a bond with the … in aqueous solution can result in hydrolysis of the maleimide. The …