Mass spectrometry-based protein footprinting for higher-order structure analysis: fundamentals and applications

XR Liu, MM Zhang, ML Gross - Chemical reviews, 2020 - ACS Publications
… Besides the covalent peptide bonds that assemble amino … to optimize the quenching
conditions to permit high sequence … , preempting useful sequence-specific fragmentation. This …

Engineering functional DNA-protein conjugates for biosensing, biomedical, and nanoassembly applications

D Zhao, Y Kong, S Zhao, H Xing - DNA Nanotechnology: From Structure to …, 2020 - Springer
… of DNA–protein conjugates for the construction of a variety of functional and dynamic
nanostructures, from isolated hybrid cages to three-dimensional (3D) protein crystalline lattices. …

Investigating polyoxometalate–protein interactions at chemically distinct binding sites

TJ Paul, TN Parac-Vogt, D Quiñonero… - The Journal of …, 2018 - ACS Publications
… Furthermore, hydrolysis of the peptide bonds of the substrate … active polyoxometalates
with improved hydrolytic activities. … nucleophile was fully optimized without any geometrical …

Protein assembly: versatile approaches to construct highly ordered nanostructures

Q Luo, C Hou, Y Bai, R Wang, J Liu - Chemical Reviews, 2016 - ACS Publications
… hot spots in protein complexes for protein engineering applications. … to highly sequence-specific
DNA binding proteins in the … His 6 -tag, a traditional ligand for Ni 2+ coordination, can be …

Protein‐detecting microarrays: current accomplishments and requirements

K Tomizaki, K Usui, H Mihara - ChemBioChem, 2005 - Wiley Online Library
… Both reviews suggest that further study involving optimization … the spectrum of
sequence-specific protein binding sites in … exchange reaction to form an amide bond and were …

A global review on short peptides: frontiers and perspectives

V Apostolopoulos, J Bojarska, TT Chai, S Elnagdy… - Molecules, 2021 - mdpi.com
… a peptide by selectively substituting along the sequence specific … stability of peptides, due
to hydrolysis of amide bonds, … The geometry optimization of short peptide can be carried out …

[图书][B] Engineering the genetic code: expanding the amino acid repertoire for the design of novel proteins

N Budisa - 2006 - books.google.com
… In order to chemically create a native amide bond between … in a target sequence [38] that
the term ‘‘protein engineering’’ … Proper calibration (optimization) of the fermentation conditions …

Optimization of ELP-Intein Protein Purification System By Comparing Four Different Self-Cleavage ELP Fusion Proteins

H Liu - 2008 - macsphere.mcmaster.ca
… -terminal splice site; (II) formation of a branched intermediate … ; (III) cleavage of amide
linkage at the intein C terminus by Asn … , two research groups used rational protein engineering

Biomacromolecule-Assisted Screening for Reaction Discovery and Catalyst Optimization

SM Ramos De Dios, VK Tiwari, CD McCune… - Chemical …, 2022 - ACS Publications
… for forming the amide bond in protein synthesis catalyzed by the … Modern protein engineering
methods allow for the … a new variant of the Jacobsen hydrolytic kinetic resolution to access …

De novo protein design, a retrospective

IV Korendovych, WF DeGrado - Quarterly reviews of biophysics, 2020 - cambridge.org
… The design of proteins that catalyze hydrolysis and oxygen-dependent reactions has …
sequence-specific recognition of helices in TM proteins can be achieved through optimization