… the amide bond between glutamine and serine residues in the … ; researchers often apply
both for protein engineering. … implanted active-site pocket are often needed for optimal packing …

… The length of the phenylethynylene linker was optimized to … a stable amide bond and is
referred to as amide (or peptide) … in protein engineering techniques, labeling proteins with small…

… Again, optimal conditions for signal amplification are a high … converges towards the catalytically
active sequence, which is … rate of hydrolysis of HPNPP. Because relying on a cascade of …

… 190 nm due to the amide chromophores of the peptide bonds. In … CD spectroscopy is widely
used in protein engineering … Sequence-Specific Labeling of Unlabeled Protein The process …

… complement experimental advances, have facilitated the understanding of protein
sequence-function relationships, and become an integral part of modern protein engineering and …

… an acylenzyme by breaking the peptide bond and the amino group leaves … 1.4 Protein
engineering of subtilisins … novel and precise amino acid sequence-specific cleavage for the use in …

… optimization of culture conditions and purification protocols in … signal sequence-specific for
Sec-dependent translocation, … of the last peptide bond in MccC7/C51 does not depend on any …

… presence of highly repetitive peptide sequences, and to the … partial hydrolysis treatment
and obtained their sequences with … Thus, their sequences are evolutionarily optimized for very …

… of a peptide bond between any two peptide fragments, a … was accomplished using two
sequence-specific zinc fingers (… Through evolutionary optimizations, the two separate precursor …

… Each peptide linkage donates one hydrogen bond and … binding peptide amyloids to promote
phosphoester hydrolysis of … in template-directed sequence-specific reactions including …