Silk, as a protein fiber characterized by high biocompatibility, biodegradability, and low toxicity, is mainly used as textile structures for various purposes, including for biological applications. The key issue for unlimited silk applicability as a modifier is to prepare its relevant form to cover or introduce to other materials. This study presents silk powder fabrication from Bombyx mori cocoons and non-dyed silk woven fabric through cryogenic milling. The cocoons were milled before and after the degumming process to obtain powders from raw structures and pure fibroin. The powder morphology and composition were analyzed using scanning electron microscopy and energy dispersive spectroscopy. The influence of the milling on the silk structure was studied using infrared and Raman spectroscopies, indicating that silk powders retained dominant β-sheet structure. The powders were also analyzed by differential scanning calorimetry and thermogravimetric techniques. The thermal endothermic peak and onset temperature characteristic for silk decomposition shifted to the lower values for all powders, indicating less thermal stability. However, the process was found to be an efficient way to obtain silk powders. The new milled form of silk can allow its introduction into different matrices or form coatings without using any harsh solvents, enriching them with new features and make more biologically friendly.