Simultaneous two-and three-photon imaging of multilayer neural activities with remote focusing
… Abstract: We present a novel remote focusing and demultiplexing scheme that allows
simultaneous two- and three-photon imaging of two-layer neural activities, featuring large
axial separation, independent foci tunability and large imaging depth enabled by three-photon
microscopy. … However, it is limited by a computationally intensive demultiplexing
algorithm as well as imaging depth limit of 2PM in a densely labeled sample. Here, we
present a novel remote focusing technique [3] to image two-layer neural activities …
simultaneous two- and three-photon imaging of two-layer neural activities, featuring large
axial separation, independent foci tunability and large imaging depth enabled by three-photon
microscopy. … However, it is limited by a computationally intensive demultiplexing
algorithm as well as imaging depth limit of 2PM in a densely labeled sample. Here, we
present a novel remote focusing technique [3] to image two-layer neural activities …
Abstract
We present a novel remote focusing and demultiplexing scheme that allows simultaneous two-and three-photon imaging of two-layer neural activities, featuring large axial separation, independent foci tunability and large imaging depth enabled by three-photon microscopy.
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