[HTML][HTML] Single-cell transcriptome profiling reveals β cell maturation in stem cell-derived islets after transplantation
Human pluripotent stem cells differentiated to insulin-secreting β cells (SC-β cells) in islet
organoids could provide an unlimited cell source for diabetes cell replacement therapy.
However, current SC-β cells generated in vitro are transcriptionally and functionally
immature compared to native adult β cells. Here, we use single-cell transcriptomic profiling
to catalog changes that occur in transplanted SC-β cells. We find that transplanted SC-β
cells exhibit drastic transcriptional changes and mature to more closely resemble adult β …
organoids could provide an unlimited cell source for diabetes cell replacement therapy.
However, current SC-β cells generated in vitro are transcriptionally and functionally
immature compared to native adult β cells. Here, we use single-cell transcriptomic profiling
to catalog changes that occur in transplanted SC-β cells. We find that transplanted SC-β
cells exhibit drastic transcriptional changes and mature to more closely resemble adult β …
Single-cell transcriptome profiling reveals β cell maturation in stem cell-derived islets after transplantation
Augsornworawat et al. demonstrate that in vitro human stem-cell-derived islets have
immature transcriptome profiles when compared to native human islets. After
transplantation, immature SC-islet cells undergo drastic changes in transcriptome and
acquire mature gene expression. Grafted SC-β cells express mature β cell genes, including
MAFA and G6PC2, resembling native human islets.
immature transcriptome profiles when compared to native human islets. After
transplantation, immature SC-islet cells undergo drastic changes in transcriptome and
acquire mature gene expression. Grafted SC-β cells express mature β cell genes, including
MAFA and G6PC2, resembling native human islets.