Abstract The AAV2. 7m8 vector is an engineered capsid with a 10-amino acid insertion in
adeno-associated virus (AAV) surface variable region VIII (VR-VIII) resulting in the alteration
of an antigenic region of AAV2 and the ability to efficiently transduce retina cells following
intravitreal administration. Directed evolution and in vivo screening in the mouse retina
isolated this vector. In the present study, we sought to identify the structural differences
between a recombinant AAV2. 7m8 (rAAV2. 7m8) vector packaging a GFP genome and its …