H₂S removal efficiency of a virgin zeolite is surveyed in this chapter by an adsorption unit fabricated in pilot scale and biogas produced directly from an anaerobic digester in ambient temperature and atmospheric pressure. The regeneration study and medium life-time were also surveyed by using it in several cycles of adsorption–desorption process. The H₂S removing efficiency of more than 90 % could be reached during the experiments. The virgin zeolite was analyzed with EDX and XRD equipments. Also the instinct regeneration ability and CH₄/CO₂ ratio-upgrading capability of zeolite are studied on this chapter. Instinct regeneration ability of the virgin zeolite showed an outstanding characteristic which makes it a promising adsorbent in industrial scale. From the results it is concluded that fresh zeolite is a suitable adsorbent for removing H₂S from biogas at ambient temperature and pressure in an economical view.