Sub-3 Å resolution structure of 110 kDa nitrite reductase determined by 200 kV cryogenic electron microscopy
N Adachi, T Yamaguchi, T Moriya, M Kawasaki… - BioRxiv, 2020 - biorxiv.org
Cu-containing nitrite reductases (NiRs), a well-studied family of 110 kDa enzymes, play
central roles in denitrification and have over 100 Protein Data Bank entries. However, such
issues as crystal packing, photoreduction, and lack of high pH cases have impeded
structural analysis of the catalytic mechanism. Here we show the cryogenic electron
microscopy (cryo-EM) structures of Achromobacter cycloclastes NiR (Ac NiR) at 2.99 and
2.85 Å resolution with pH 6.2 and 8.1, respectively. Comprehensive comparisons with cryo …
central roles in denitrification and have over 100 Protein Data Bank entries. However, such
issues as crystal packing, photoreduction, and lack of high pH cases have impeded
structural analysis of the catalytic mechanism. Here we show the cryogenic electron
microscopy (cryo-EM) structures of Achromobacter cycloclastes NiR (Ac NiR) at 2.99 and
2.85 Å resolution with pH 6.2 and 8.1, respectively. Comprehensive comparisons with cryo …
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