[HTML][HTML] The Akt/mTOR pathway assures the synthesis of HIF-1α protein in a glucose-and reoxygenation-dependent manner in irradiated tumors
H Harada, S Itasaka, S Kizaka-Kondoh… - Journal of Biological …, 2009 - ASBMB
Transcriptional activity of HIF-1 (hypoxia-inducible factor-1) has been reported to be up-
regulated in solid tumors after ionizing radiation; however, the molecular mechanism
underlying the response remains to be elucidated. In the present study, we performed a
series of molecular imaging experiments using a HIF-1-dependent reporter gene, 5HREp-
ODD-luc, and found an essential role of the Akt/mTOR pathway. Hypoxic tumor cells distant
from blood vessels were dramatically reoxygenated at 24 h postirradiation, and HIF-1 activity …
regulated in solid tumors after ionizing radiation; however, the molecular mechanism
underlying the response remains to be elucidated. In the present study, we performed a
series of molecular imaging experiments using a HIF-1-dependent reporter gene, 5HREp-
ODD-luc, and found an essential role of the Akt/mTOR pathway. Hypoxic tumor cells distant
from blood vessels were dramatically reoxygenated at 24 h postirradiation, and HIF-1 activity …