Mini-inteins derived fromSynechocystis sp.(Ssp DnaB intein) andMycobacterium xenopi
(Mxe GyrA intein) that have been modified to cleave peptide bonds at their C and N termini,
respectively, were cloned in-frame to the N and C termini of a target protein. Peptide bond
cleavage of the modified inteins generated an N-terminal cysteine and a C-terminal thioester
on the same protein. These complementary reactive groups underwent intra-or
intermolecular condensation to generate circular or polymeric protein species with a new …