The present study aims at highlighting the major role of ecological agriculture in the current organic farming context, internationally and nationally, and the importance of using natural fertilizers in the vegetable culture. The research was conducted at the Banu Mărăcine Didactic Station-Romania, in unheated solarium, on some cultivars of tomatoes (Antalya, Cemil and Lorely). There was observed the impact of foliar treatment with three different biofertilizers consisting of humic acids extracted from brown coal, humic acids mixed with polyphenolic extract of Vitis vinifera seeds and humic acids mixed with polyphenolic extract of Vitis vinifera seeds and Boron, on the content of chlorophyll, total carotene on stages of plant and fruit development and obtained production. It was found that the biochemical changes and obtained production vary depending on the nature of the fertilizer used and of the cultivar, the best results occurring in the variant fertilized with humic acids+ extract from the Vitis vinifera seeds+ Boron (HA+ ESVv+ B).