Myocilin is a protein found in the trabecular meshwork extracellular matrix tissue of the eye
that plays a role in regulating intraocular pressure. Both wild-type and certain myocilin
variants containing mutations in the olfactomedin (OLF) domain are linked to the optic
neuropathy glaucoma. Because calcium ions are important biological cofactors that play
numerous roles in extracellular matrix proteins, we examined the calcium binding properties
of the myocilin OLF domain (myoc-OLF). Our study reveals an unprecedented high affinity …