Information literacy is elemental to quality library services, the knowledge of which will improve the service delivery and attract the members of the academic community to the library. The study examined perception of information literacy skill among the librarians using University of Lagos library as a case study. The study focused on three research questions and hypotheses to elucidate the opinion of library on information literacy and how it has helped the effectiveness of librarians. The study adopted postpositivist research paradigm in which quantitative and qualitative research method were used, data was gathered through questionnaires and content analysis. The target population were librarians and library officers who are purposively selected from the University of Lagos Library. The study established that Librarian and Library Officers possessed advanced level of perception of the need for information literacy, used library resources effectively; age group has significant effect on information literacy, librarians with higher qualification use library resources more than librarians with lower qualification and the level of information literacy of librarians also determine the extent of use of library resources. Female librarians are significantly more than their male counterparts. The recommendations focused on how issues and challenges could be approached positively to lead to better service delivery for a cosmopolitan environment like Lagos.