The development of local bull in many regions is currently leading to the development of local bulls and Indonesia is no exception, especially in South Sulawesi Province. One of local bulls in which developed for approximately last ten years is Bali-polled bull. Thus, the selection of polled bulls is crucial, mainly in modern livestock management. The aims of this study to identify sperms post-thawing quality and proteomic plasma semen as fertility performance in Bali-polled bull. The utilization of Bali-horned bulls as a point of reference was necessary to achieve this aim. The semen samples from Bali-horned and Bali-polled bulls were obtained twice weekly using an artificial vagina. The semen samples were immediately sent to the laboratory for processing. Parameters measured were sperm motility, abnormality, viability, acrosome integrity, and membrane integrity. Liquid chromatography-mass spectrometry (LCMS/MS) analysis was used to assess the confirmation profile protein of seminal plasma. The conception rate from artificial insemination was used to determine the fertility rate (AI). This investigation revealed that the quality of the sperms from Bali-polled bulls and Bali-horned bulls did not substantially change after thawing (p> 0.05). However, sperm acrosome integrity was considerably (p< 0.05) higher in Bali-polled bulls than in Bali-horned bulls. In the profiling protein seminal plasma, ZPBP protein expression was found in the Bali-polled bull which was not found in the Balihorned bull. Bali-polled bulls and Bali-horned bulls did not differ significantly (p> 0.05) in terms of fertility rate on conception rate. In conclusion, sperms post-thawing quality of Bali-polled bull have a good category according SNI 4869-1: 2017 and proteomic seminal plasma of Bali-polled bull has a protein profile linked to reproductive function.