Transcription of estrogen receptor α and β in mouse cerebral cortex: effect of age, sex, 17β-estradiol and testosterone
Estrogen actions are mainly mediated by estrogen receptor (ER) α and ERβ which in turn
are regulated by several factors including age, sex and gonadal steroid hormones 17β-
estradiol and testosterone. In the present study, we have used nuclear run-off assay to
examine the effect of these factors on the rate of transcription of ERα and ERβ of mouse
cerebral cortex. The run-off assay result was further corroborated with the measurement of
steady state level of ERα and ERβ mRNA by semiquantitative RT-PCR method. Our results …
are regulated by several factors including age, sex and gonadal steroid hormones 17β-
estradiol and testosterone. In the present study, we have used nuclear run-off assay to
examine the effect of these factors on the rate of transcription of ERα and ERβ of mouse
cerebral cortex. The run-off assay result was further corroborated with the measurement of
steady state level of ERα and ERβ mRNA by semiquantitative RT-PCR method. Our results …
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