Trichostatin A improves preimplantation development of bovine cloned embryos and alters expression of epigenetic and pluripotency genes in cloned blastocysts
HJ Oh, TH Lee, JH Lee, BC Lee - Journal of Veterinary Medical …, 2012 - jstage.jst.go.jp
We investigated the effects of exposure time and concentration of trichostatin A (TSA) on in
vitro development and quality of bovine SCNT embryos. At multiple time points, the relative
expression of genes related to pluripotency and reprogramming was analyzed in order to
assess the quality of preimplantation embryos cultured in media with TSA using real-time
PCR. Development into blastocysts was higher in 100 nM TSA than in controls (35.96 vs.
28.30%, P< 0.05). Study of 100 nM TSA exposure time showed development into blastocysts …
vitro development and quality of bovine SCNT embryos. At multiple time points, the relative
expression of genes related to pluripotency and reprogramming was analyzed in order to
assess the quality of preimplantation embryos cultured in media with TSA using real-time
PCR. Development into blastocysts was higher in 100 nM TSA than in controls (35.96 vs.
28.30%, P< 0.05). Study of 100 nM TSA exposure time showed development into blastocysts …
Trichostatin a improves preimplantation development of bovine cloned embryos and alters expression of epigenetic and pluripotency genes in cloned blastocysts.
OHJ Oh HyunJu, TH Lee, JH Lee… - 2012 - cabidigitallibrary.org
We investigated the effects of exposure time and concentration of trichostatin A (TSA) on in
vitro development and quality of bovine SCNT embryos. At multiple time points, the relative
expression of genes related to pluripotency and reprogramming was analyzed in order to
assess the quality of preimplantation embryos cultured in media with TSA using real-time
PCR. Development into blastocysts was higher in 100 nM TSA than in controls (35.96 vs.
28.30%, P< 0.05). Study of 100 nM TSA exposure time showed development into blastocysts …
vitro development and quality of bovine SCNT embryos. At multiple time points, the relative
expression of genes related to pluripotency and reprogramming was analyzed in order to
assess the quality of preimplantation embryos cultured in media with TSA using real-time
PCR. Development into blastocysts was higher in 100 nM TSA than in controls (35.96 vs.
28.30%, P< 0.05). Study of 100 nM TSA exposure time showed development into blastocysts …
以上显示的是最相近的搜索结果。 查看全部搜索结果