3D Direct laser writing (3D DLW) based on two photon polymerization represents a powerful technique for the additive manufacturing of 3D micro- and nano-structures, which have a number of promising applications in areas including biology and cell cultures up to soft robotic. New applications often entail the contact and movement of nanoscale areas, therefore understanding of the mechanical properties involved in such systems is necessary for creating reliable micro and nanoscale applications. Notably, knowledge about nanomechanics of 3D printed structures and its dependence on its geometry and contact size remains essential. This study focuses on the investigation of the Young’s modulus of 3D printed microstructures by Atomic Force Microscopy. Using the PeakForce QNM AFM mode, it was possible to determine the influence of the laser power and the monomer and their mixture on the micromechanical properties, in particular the Young's modulus. The fabricated structures are 2D structures, consisting of successive lines forming a square. Three different resins have been used based on poly(ethyleneglycol)diacrylate (PEGDA), trimethylolpropane triacrylate (TMPTA) and pentaerythritol triacrylate (PETA). The results obtained show that the increase in laser power increases the Young's modulus of these raw materials, since it induces a stronger crosslinking density. Mixtures of resins have also been formulated inducing a Young's modulus structure increase when increasing the amount of PETA. By combining these two approaches, tuning of the nanomechanical properties of the final microstructures on an unprecedented range covering 3 orders of magnitude, from MPa up to GPa have been successfully achieved. This major result paves the way to in-depth reflection on future works in nanorobotics or biomedical devices, where complex mechanical behavior are highly desired.