[HTML][HTML] Tylophorine abrogates G2/M arrest induced by doxorubicine and promotes increased apoptosis in T47D breast cancer cells
Asian Pacific journal of cancer prevention: APJCP, 2018•ncbi.nlm.nih.gov
Background: The effects of tylophorine, a natural alkaloid found in Tylophora indica,
administered as a single compound or in combination with doxorubicin on cell cycling and
apoptosis were assessed in T47D breast cancer cells, selected as a model system for breast
cancer. Methods: Cell cycle distribution and apoptosis were examined by flow cytometry.
Caspase 3 and 9 expression was determined by immunocytochemistry. Result: We found
that tylophorine did not significantly influence the cell cycle distribution of T47D cells …
administered as a single compound or in combination with doxorubicin on cell cycling and
apoptosis were assessed in T47D breast cancer cells, selected as a model system for breast
cancer. Methods: Cell cycle distribution and apoptosis were examined by flow cytometry.
Caspase 3 and 9 expression was determined by immunocytochemistry. Result: We found
that tylophorine did not significantly influence the cell cycle distribution of T47D cells …
Abstract
Background:
The effects of tylophorine, a natural alkaloid found in Tylophora indica, administered as a single compound or in combination with doxorubicin on cell cycling and apoptosis were assessed in T47D breast cancer cells, selected as a model system for breast cancer.
Methods:
Cell cycle distribution and apoptosis were examined by flow cytometry. Caspase 3 and 9 expression was determined by immunocytochemistry.
Result:
We found that tylophorine did not significantly influence the cell cycle distribution of T47D cells. However, the alkaloid did prevent accumulation of cells in the G2/M phase. In addition, tylophorine increased the number of apoptotic cells. Expression of proapoptotic proteins (caspases 3 and 9) was up-regulated upon administration of tyloporine alone or in combination with doxorubicin.
Conclusions:
Tylophorine alone or in combination with doxorubicin induced apoptosis in T47D breast cancer cells through modulation of the cell cycle and affecting the expression of caspases 3 and 9.
ncbi.nlm.nih.gov
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