Using doubly-labelled water to measure free-living energy expenditure: some old things to remember and some new things to consider
JR Speakman, C Hambly - … Biochemistry and Physiology Part A: Molecular …, 2016 - Elsevier
The doubly-labelled water (DLW) method provides the ability to measure the energy
expenditure of free-living animals based only on the injection of two isotopes in water (one
of oxygen and one of hydrogen) and traditionally the collection of 2 blood samples. We
review here the fundamental basis of how the method works, and highlight how the choice of
the appropriate calculation equation can have a large impact on the resultant estimates,
particularly in species where the difference between the isotope elimination constants is …
expenditure of free-living animals based only on the injection of two isotopes in water (one
of oxygen and one of hydrogen) and traditionally the collection of 2 blood samples. We
review here the fundamental basis of how the method works, and highlight how the choice of
the appropriate calculation equation can have a large impact on the resultant estimates,
particularly in species where the difference between the isotope elimination constants is …
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