Due to inadequate intake of Vitamin D, identification of individuals characterised by the highest risk of deficiencies is one of the more crucial tasks for public health. The aim of the presented study was to assess the validity and reproducibility of the designed Vitamin D dietary intake questionnaire based on food frequency assessment—VIDEO-FFQ (VItamin D Estimation Only—Food Frequency Questionnaire) in a group of Polish women aged 20–30 years. Seventy-five participants kept a three-day dietary record and filled out the VIDEO-FFQ twice (immediately after the three-day dietary record and after six weeks). The assessment of validity and reproducibility was conducted by verifying standard errors of estimation, median differences, and percentages of individuals classified into tertiles, correlations and Bland-Altman plots. The Vitamin D intake for the majority of the surveyed women was inadequate as over 85% of them were characterised by values of intake lower than 5.0 μg per day. The results allowed concluding that a high accuracy of the VIDEO-FFQ was achieved. The required Bland-Altman index values lower than 5.0% were obtained, confirming satisfactory validity and reproducibility. The VIDEO-FFQ may be deemed a convenient practical tool for the estimation of Vitamin D intake in young women.