Anredera cordifolia (Ten.) Steenis, also known as Binahong, is a family of Basellaceae, a medicinal plant that had been growing well for many years. Binahong leaf extract can stimulate fibroblast and collagen formation which will accelerate the process of wound healing. This study was carried out to observe the proliferation kinetics and migratory activity of 3T3 fibroblast cell lines induced by Anredera cordifolia (Ten.) Steenis ethanol extract. Anredera cordifolia (Ten.) Steenis powder was extracted by maceration with 70% ethanol. Proliferation activity was assessed by using the MTT assay. Cell migration activity was determined using scratch assay. The data obtained were analyzed statistically with data analysis, descriptive analysis, and continued with the bivariate analysis of correlation between case groups. The results of phytochemical analysis test on the content of Binahong leaf extract’s secondary metabolites confirmed the presence of saponins, tannins, alkaloids, triterpenoid, flavonoid, phenolics, steroids, and glycosides. Fibroblast cell proliferation was increased from 0 h until 72 h with the highest proliferation rate at the concentration of 62.5 µg/mL (127.89±16.12). The results showed that there was a significant difference in the mean proliferation rate of fibroblast cells between the Binahong leaf extract, Aloclair® and the control group (p value= 0.0001). Anredera cordifolia (Ten.) Steenis leaf extract has chemical compounds, such as saponins, tannins, alkaloids, triterpenoid, flavonoid, phenolics, steroids, and glycosides. It is potentially effective as a wound healing agent which stimulates proliferation of fibroblast cells.