… of the BCR gene are spliced to exon 2 of the ABL gene. Amplification of the chimeric BCR-ABL
mRNA by the reverse/transcription polymerase chain reaction (RT-PCR) is a very …

… gene (e1) and the third exon of ABL gene (a3). The choice of reverse primers located on
ABL exon 3 rather than on ABL … -ABL junction takes place between the BCR exon 1 and ABL …

… The comparison of real-time PCR and the competitive … BCR-ABL kinetics, are closely similar
to those of BCR-ABL/B2M by … total-ABL should not be used as a control gene in BCR-ABL …

… ABL fusion gene. We set up and evaluated a rapid and reliable real-time reversetranscription-polymerase
chain reaction (RT-… probes used for real-time RTPCR assay for amplification of …

… 5′ region of the BCR gene fused to the second exon of the ABL gene. The resulting hybrid
… BCR gene within the breakpoint cluster region, 6 and Western blotting to detect the Bcr-Abl …

… The fused BCR/abl gene and its gene products provide … real-time PCR(Q-Rt-PCR) in
the detection of BCR/abl fusion … with conventional reverse transcription-mediated PCR (RT–PCR). …

… (b) and (c) Amplification of total RNA with BCR-ABL gene primers: first-round PCR (b), the
PCR product presented a size of 392 or 317 bp, respectively, for the b3a2 and b2a2 transcripts…

… gene product as … -ABL positive patients who are at high risk of consecutive leukemic relapse.
We further asked whether the amount of BCR-ABL that was assessed by the real-time PCR …

… The BCR-ABL gene can be detected using several molecular methods, ie, … reverse-transcription
polymerase chain reaction (RT-PCR). For its simplicity, rapidity, and sensitivity, RT-PCR …

… reverse transcriptasepolymerase chain reaction (RT-PCR) provides a powerful tool for
minimal residual CML detection. The RT-PCR … involved in the reverse transcription reaction to …