… and challenges in method development and validation for the absolute quantification of endogenous biomarker metabolites using liquid chromatographytandem …

MM Khamis, DJ Adamko… - Mass spectrometry …, 2021 - Wiley Online Library
… Although a section was dedicated to endogenous metabolites absolute quantification (ICH, …
-MS/MS methods intended for the absolute quantification of endogenous metabolites. It is not …

Absolute quantification of monoclonal antibodies in biofluids by liquid chromatographytandem mass spectrometry

C Hagman, D Ricke, S Ewert, S Bek… - Analytical …, 2008 - ACS Publications
… Further similar efforts included work on the quantification of the G-protein-coupled receptor
… the absolute quantification of yeast proteins involved in gene silencing. The quantification of …

Ultra performance liquid chromatography isotope dilution tandem mass spectrometry for the absolute quantification of proteins and peptides

LG Luna, TL Williams, JL Pirkle, JR Barr - Analytical chemistry, 2008 - ACS Publications
… Similar HPLC methods have been applied to the absolute quantification of peptides and
proteins. These quantification methods are often run on large 2.1- or even 4.6-mm-id columns, …

Liquid chromatography/tandem mass spectrometry assay for the absolute quantification of the expected circulating apelin peptides in human plasma

C Mesmin, M Dubois, F Becher… - … in Mass Spectrometry, 2010 - Wiley Online Library
… As standard immunoassays cannot specifically quantify each apelin peptide, we have
developed a sensitive and targeted multiplexed liquid chromatography/tandem mass spectrometry …

Simultaneous absolute quantification of 11 cytochrome P450 isoforms in human liver microsomes by liquid chromatography tandem mass spectrometry with in silico …

H Kawakami, S Ohtsuki, J Kamiie, T Suzuki… - Journal of …, 2011 - Elsevier
quantify 11 CYP isoforms by multiplexed-multiple reaction monitoring analysis with liquid
chromatography tandem … The established quantification method detected target peptides at 10 …

Absolute quantification of multidrug resistance-associated protein 2 (MRP2/ABCC2) using liquid chromatography tandem mass spectrometry

N Li, OV Nemirovskiy, Y Zhang, H Yuan, J Mo, C Ji… - Analytical …, 2008 - Elsevier
… In the present study, an absolute quantification method was … The lower limit of quantification
was established to be 31.25 … Using this method, we successfully determined the absolute

Absolute quantification of aldehyde oxidase protein in human liver using liquid chromatographytandem mass spectrometry

JT Barr, JP Jones, CA Joswig-Jones… - Molecular …, 2013 - ACS Publications
… Herein, we developed and employed a trypsin digest and subsequent liquid
chromatographytandem mass spectrometry analysis to determine absolute amounts of AOX1 in …

Absolute quantification of proteins and phosphoproteins from cell lysates by tandem MS

SA Gerber, J Rush, O Stemman… - Proceedings of the …, 2003 - National Acad Sciences
… of reverse-phase chromatographic retention time. The absolute quantification is determined
by … Because an absolute amount of the AQUA peptide is added (eg, 250 fmol), the ratio of the …

Development of an absolute quantification method targeting growth hormone biomarkers using liquid chromatography coupled to isotope dilution mass spectrometry

S Kirsch, J Widart, J Louette, JF Focant… - … of Chromatography A, 2007 - Elsevier
… A method to perform absolute quantification of two biomarkers (IGF-1 and IGFBP-3) of …
of target proteins are analysed by liquid chromatographytandem mass spectrometry (LC–MS/MS) …

The absolute quantification strategy: a general procedure for the quantification of proteins and post-translational modifications

DS Kirkpatrick, SA Gerber, SP Gygi - Methods, 2005 - Elsevier
… sequencing experiments performed by liquid-chromatography tandem mass spectrometry
(LC–… the same physicochemical properties including chromatographic co-elution, ionization …